Screening and
Identification of Tumor Related Genes in Early Stages of Transformed NIH3T3
Cells Associated with Overexpressed PTP¦Á
YANG Jin-Song, ZHENG Xin-Min, CHEN
Shi-Shu*
( Department of Biochemistry, Laboratory of Molecular Biology and
Research Center for Human Gene Therapy, Shanghai Second Medical University,
Shanghai 200025, China )
Abstract An inducible-PTP¦Á
expression system was used to screen differential expression genes in NIH3T3
cells overexpressing PTP¦Á, which could be used as a model for studying
mechanism of tumorigenesis at early stage. After PTP¦Á induction for 24 h, 65
differential bands were obtained, among which, there were 29 known genes, 12
known EST, 6 novel EST by bioinformatic analysis. Functions of the known genes
were related to signal transduction, structural/adhesion, energy metabolism,
transcription/translation, ribosome and apoptosis. The differential expression
of the genes coding for G protein, TCR, Trx-related protein, caveolin-1 was
verified by RT-PCR and Northern blot. The results showed that the expression of
numerous genes was changed after the induction of PTP¦Á for 24 h, these genes
are related to many cellular physiological functions, and some of them play
important roles in the early stage of tumorigenesis. The results provide basis
for further exploring the molecular mechanism of tumorigenesis and these genes
may well be candidate targets for gene therapy.
Key words PTP¦Á; differential expression; mechanism of
tumorigenesis
*Corresponding author: Tel,
86-21-63846590-776452; e-mail, [email protected]